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. 2016 Oct 5;204(4):1407–1422. doi: 10.1534/genetics.116.195099

Figure 3.

Figure 3

top-2(it7ts) embryos lack paternal DNA. Wild type (OCF8) and top-2(it7ts) hermaphrodites were shifted to 24° as L4s. After 24 hr, hermaphrodites were dissected and the embryos imaged. Red, chromatin (mCherry::H2B); Green, tubulin (GFP::TBA-2) and nuclear envelope (NPP-1::GFP). White triangle indicates paternal DNA. Dashed-line circle demarks area of missing paternal DNA. Two example images of pronuclear meeting are given. The number of wild-type embryos observed was 48. The number of top-2(it7ts) embryos observed was 47. Bar, 30 μm.