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DNA content and budding in cln1 cln2 cln3 CLB3∆db cells after shutoff of MET3-CLN2. (A) Percent unbudded cells after block of MET3-CLN2 by addition of methionine. First sample (t = 0) was taken before addition of methionine. (B) DNA content after block of MET3-CLN2.
