Figure 9.

Testing bypass of CLNs and CLB5/6 in CLB3∆db strains. (A) Serial dilutions of CLB3∆db/wt strains with a cln1 cln2 cln3 MET3-CLN2 background. Cells were grown to log-phase in medium lacking methionine, then plated in 10-fold serial dilutions on solid medium containing methionine to shut off MET3-CLN2. (B) CLB3∆db/wt strains with a clb5 clb6 cln1 cln2 cln3 MET3-CLN2 background were plated in 10-fold serial dilutions as in (A). (C) cln- cells with either wild-type, CLN2, CLB3∆db, sic1, or whi5 backgrounds were plated in 10-fold serial dilutions as in (A).