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. 2016 Oct 3;10(6):444–465. doi: 10.1080/19336896.2016.1234574

FIGURE 4.

FIGURE 4.

Expression of Hsp31 in combination with Hsp104 increases prion curing. (A) Hsp31 and p426-GPD-Hsp104 were co-transformed in the [PSI+] strain. Vector without inserts served as controls. (B) Quantification of the experiments in panel A. Prion curing was increased from about 2.5% to 6% when Hsp31 was co-expressed with Hsp104 compared to the control strain (*unpaired Student's t-test; p ≤ 0.001, n = 3). (C) [PSI+] hsp31Δ strain harboring plasmids for Hsp104 and Hsp31. (D) Quantification of experiments describe in panel C. The combination of Hsp104 and Hsp31 increased prion curing in the [PSI+] hsp31Δ strain consistent with WT strain in A-B. (** One-way ANOVA; p ≤ 0.001, n = 3). (E) Image of p426-GPD-Hsp42 transformed cells demonstrating curing compared to vector only. (F) Hsp31 and p426-GPD-Hsp42 were co-transformed in the [PSI+] strain and quantified. The combination of Hsp42 and Hsp31 did not increase curing (ns = not significant). Data and images shown are representative of at least 3 independent biological experiments for all panels.