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. 2016 Dec 16;11(12):e0167622. doi: 10.1371/journal.pone.0167622

Fig 1. Expression of TRPV1 in human testis.

Fig 1

A) Analysis of the expression of TRPV1 mRNA in human testis biopsies (Hum. testis) and ejaculated human spermatozoa (Hum. sperm) by RT-PCR. Data are reported as normalized relative quantification (nRQ). Relative expression was also reported as gel electrophoresis analysis of the target amplification product at 186 base pairs (TRPV1) and GAPDH as housekeeping gene (108 base pairs). B) Analysis of the protein expression of TRPV1 in human testis biopsies (Human testis) and ejaculated human spermatozoa (Human sperm) by western blot. The specificity of primary immunoreaction was assessed by co-incubation with the immunogen peptide (Peptide). Relative expression was reported as the ratio between the band density at ~90 kDa (TRPV1) and the band density of β-Actin as housekeeping (43 kDa). Images are representative of six independent experiments. Significance: * = P<0,05 vs human testis; ǂ = P<0,05 vs human sperm. C) Evaluation by immunofluorescence of TRPV1 protein localization (green) in whole human seminiferous tubule, testis cell populations from fine needle aspiration specimens (D) and ejaculated spermatozoa (E). Samples were counterstained with DAPI (blue). In the negative control (Neg insert in C) primary antibody was omitted. As additional control condition, immunogen peptide was added in the reaction mixture (+ Peptide).