Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Neurobiol Dis. 2016 Sep 19;96:248–260. doi: 10.1016/j.nbd.2016.09.013

Figure 5. Effects of TH on chemokines activity and microglia migration after OGD in neuronal and BV2 microglial cell cultures.

Figure 5

The cooling effects on inflammatory responses were examined in cultured neuronal and BV2 microglial cells subjected to OGD. Physical cooling was achieved using a hypothermic incubator (33°C, 5% CO2) during OGD. A. OGD led to significant neuronal death, but the increase was attenuated by hypothermia. # P<0.05 versus no OGD groups; * P<0.05 versus OGD control group; n=3 per group. B. MCP-1 and MIP-1α mRNA levels in control (CTL) and hypothermia (cooling) groups, as determined by RT-PCR analysis. Reducing the medium temperature to 33°C during OGD decreased MCP-1 and MIP-1α increases in neuronal cells. # P<0.05 versus no OGD groups; * P<0.05 versus OGD control group; n=3 per group. C. Representative photographs of the transmigrated Hoechst 33342-labeled microglia under each condition in a Transwell chamber. Scale bars = 20 µm. D. Quantified graphs of the number of the transmigrated microglia in co-cultivation of neuronal and/or BV2 microglial cells. # P<0.05 versus no OGD groups; * P<0.05 versus OGD control group; n=3 per group.