Supplemental Fig. 1.

Parasite growth rates in RBCs from children categorized by different definitions of anemia at baseline. In analysis of parasite growth rates in RBCs from children at Day 0, we stratified participants (all anemic) using four different definitions to categorize the severity and type of iron deficiency in the presence or absence of inflammation: those with 1) hepcidin < 5.5 ng/ml (n = 82); 2) ferritin < 12 ng/ml (n = 69); 3) ferritin12-30 ng/ml with CRP > 5 mg/ml (n = 17); 4) hemoglobin increase of > 0.5 g/dl from baseline after 49d or 84d of daily iron supplementation (n = 46); definitions 1–4 are not necessarily mutually exclusive. Of note, everyone in our population had a raised serum transferrin receptor (sTfR):log ferritin index > 2 which is highly suggestive of iron deficiency. Growth rate values are presented relative to growth in RBCs from non-anemic donors. Each dot represents the mean result of triplicate growth assays from each donor and the error bars represent 95% CI. Mean growth rate results (with 95%CI) are: hepcidin < 5.5 ng/ml = 42.89% (37.11–48.67%); ferritin < 12 ng/ml = 43.34% (36.01–50.68%); ferritin 12-30 ng/ml with CRP > 5 mg/ml = 49.08% (29.16–69.00%), ΔHgb > 0.5 g/dl = 44.04% (35.14–52.93%). There are no significant differences between the means.