Supplemental Fig. 3.

Changes in parasite growth, invasion, and reticulocytosis in RBCs from anemic children before and after daily iron supplementation.

a) Levels of parasite growth rates increase over time in anemic children undergoing iron supplementation, as depicted by line graph in order to highlight changes for each individual that had data available at all timepoints (n = 35 children with complete repeat growth measures at Day 0, 49, and 84, with 86% having increased growth rate at Day 49) One-way repeated measures ANOVA of growth rate values indicates the means are significantly different between Days (p < 0.0001); post-hoc analysis with Tukey's test indicates significant differences between Day 0 and Day 49 means (p < 0.001) and Day 49 and Day 84 means (p < 0.001), but no significance between Day 0 and Day 84 for those children with repeat measures.

b) Direct comparison of invasion into RBCs from non-anemic donors to RBCs from 8 anemic children either before or during 12 mg daily iron supplementation. Each experiment was performed in triplicate for each blood donor. The marker represents the SI point estimate and the bar represents the 95% CI. An SI of 1.0 indicates no difference in parasite invasion of the two RBC populations. Student's t-test indicates significant differences between pre- and post-iron SI values (**p < 0.01).

c) Line graph of CD71 repeated measures (n = 31 children with complete repeat CD71 measures at Day 0, 49, and 84). In 21 of these children, the relative percent CD71 positive cells increased from Day 0 to Day 49. See Fig. 3B for repeated measures ANOVA statistics.