(A and B) Representative TEM images of liposomes (A) and resulting LGNP (B) according to filter pore size (30, 50, 100, and 200 nm). (C and D) Size distributions (C) and relation (D) between the liposomes and the LGNP. (E) Representative absorbance spectra of the LGNP solutions with different sizes. a.u., arbitrary units. (F) Plot of absorbance at SPR peak (541 nm) of the LGNP as a function of time. (G and H) Representative TEM image (G) and EDS spectrum (H) of the LGNP prepared by using ascorbic acid as another reducing agent. (I) Concentrations of gold precursor in the liposome due to diffusion after 0, 80, and 160 μs. (J) The number of gold atoms in the liposome due to reduction after 0, 20, and 40 hours. (K) Concentration of gold precursor (black) and the number of gold atoms (red) inside the liposome as a function of time. Scale bars, 50 nm (A, B, and G) and 10 nm [insets in (B), (G′), (I), and (J)].