Fig. 1.

Cytotoxicity of ARS4 against human ovarian normal and cancer cells.

(a) The design strategy and chemical structure of ARS4. (b) IC₅₀ values of ARS4 and its parent compounds, DHA and melphalan, for human ovarian cancer cells, A2780 and OVCAR3, and human ovarian epithelial cells, IOSE144. Cytotoxicity was assessed by CCK8 assays after 48 h of incubation with the indicated compounds. Viability of A2780 (c) and OVCAR3 cells (d) after exposure to ARS4 and its parent drugs DHA and melphalan at various concentrations for 48 h. Inhibition of proliferation of A2780 (e) and OVCAR3 (f) cells after exposure to ARS4 for 0, 24, 48, or 72 h.