Video 1. Transcriptional activation of CCND1 in response to Wnt3a.
HEK293 CCND1-MS2 cells stably expressing MS2-GFP (green) were treated with Wnt3a. The transcribed CCND1 mRNA on the active gene is seen as a bright green dot. The fluorescent signal on the active genes was enhanced using ImageJ 'Spot Enhancing Filter 2D' in order to clearly present the active sites in the movie. Cells were imaged every 15 min for 6 hr.