Video 11. YFP-β-catenin puncta move from the membrane to the centrosome.
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DOI: 10.7554/eLife.16748.027
HEK293 CCND1-MS2 cells stably expressing YFP-β-catenin were treated with Wnt3a and MG132. At the 165 min time point, a series of YFP-β-catenin puncta can be tracked (track colors) moving from the membrane to the centrosome. The YFP signal is pseudo-colored using ImageJ ‘Green Fire Blue’ look-up table to show YFP-β-catenin levels. Cells were imaged every 15 min for 1065 min.