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HEK293 CCND1-MS2 cells stably expressing YFP-β-catenin were treated with Wnt3a, and the dynamics of the protein were observed in individual cells. The YFP signal is pseudo-colored using ImageJ ‘Green Fire Blue’ look-up table to show YFP-β-catenin levels. Cells were imaged every 15 min for 825 min.
