Video 8. YFP-β-catenin dynamics at the cell membrane following Wnt3a activation.
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DOI: 10.7554/eLife.16748.021
HEK293 CCND1-MS2 cells stably expressing YFP-β-catenin were treated with Wnt3a, and the dynamics of the protein at the membrane were followed over time, and were similar to the nucleus and cytoplasm accumulation. The YFP signal is pseudo-colored using ImageJ ‘Green Fire Blue’ look-up table to show YFP-β-catenin levels. Cells were imaged every 15 min for 1065 min.