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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Virol Methods. 2016 Mar 29;233:62–71. doi: 10.1016/j.jviromet.2016.02.016

Figure 2. Effects of SiaNAz incorporation and click labeling on pseudovirus infectivity.

Figure 2

HIV-1 particles pseudotyped with HIV-1 HXB2 Env (A, D), VSV-G (B, E) and ASLV Env (C, F) were produced in 293T cells in the absence or in the presence of varied concentrations of Ac4ManNAz (ManNAz). Virus infectivity was evaluated by a reporter luciferase assay using 1 ng of viral p24, as determined by ELISA, before (A–C) and after (D–F) labeling with indicated concentrations of Alexa488-DIBO, as described in Methods. Data are means and standard deviations from triplicate experiments.