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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Virol Methods. 2016 Mar 29;233:62–71. doi: 10.1016/j.jviromet.2016.02.016

Figure 4. Fusion of single VSVpp co-labeled with Alexa488-DIBO (green) and Gag-imCherry (red).

Figure 4

(A) Images of two double-labeled VSVpp fusing with endosomes of CV-1-derived cells nearly at the same time, as manifested in change from colocalized yellow color to green color. Scale bar 2 μm. (B) Sum fluorescence intensity profiles for the lower particle in panel A obtained by single particle tracking. (C, D) images and fluorescence profiles for a control double-labeled particle that did not undergo fusion.