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A. L90M mutation in PRS17 (green) induces shortened C-H…O interaction between Met90 and catalytic Asp25 in comparison to wild-type PR (gray). I93L mutation in PRS17 results in loss of van der Waals contact observed between Ile93 and Leu 90 of wild-type PR. B. Distal mutations A71V and I93L in PRS17 are associated with shift in 70’s β strand by ~1 Å and loss of the ion pair between His69 and the carboxylate tail of the second subunit.
