Table 2. Crossing-point or threshold cycle used to differentiate between infection and colonization.

Bacterial count	Crossing point	Se (95%CI)	Sp (95%CI)	PPV (95%CI)	NPV (95%CI)	PLR (95%CI)	NLR (95%CI)
≥105 cfu/mL	≤24.7	94.7 (82.1–100.0)	94.7 (88.1–100.0)	85.7 (68.4–100.0)	98.2 (93.7–100.0)	18.00 (5.95–54.43)	0.06 (0.01–0.37)
<104 cfu/mL	>26.9	84.2 (65.2–100.0)	100.0 (98.7–100.0)	100.0 (96.9–100.0)	92.7 (83.5–100.0)	Undefined	0.16 (0.06–0.45)

Se: sensitivity; Sp: Specificity; PPV: positive predictive value; NPV: negative predictive value; PLR: positive likelihood ratio; NLR: negative likelihood ratio; cfu: colony forming units.