Fig 9. Effect of different concentrations of norbixin on the accumulation of A2E by porcine RPE cells in vitro.

RPE cells were treated with norbixin (or DMSO) and with A2E but not illuminated and cells were extracted with MeOH. A2E concentrations are expressed as absolute amounts per 10⁵ cells. Bars represent the mean ± s.e.m. of three separate experiments with n = 3. **p<0.01, ***p<0.001 compared to cells non treated with norbixin (One-way ANOVA, Dunnett’s post test).