TABLE 6.
Relative adiponectin concentrations and relative adiponectin and ALDH1A1 mRNA expression in 3T3-adipocytes
| Adiponectin | Significance | |||||
|---|---|---|---|---|---|---|
| Retinoid | ELISA | REX | ALDH1A1 REX | ELISA | Adiponectin REX | ALDH1A1 REX |
| ATRA | 0.57 ± 0.14 | 0.25 ± 0.03 | 3.19 ± 0.07 | 0.02 | <0.01 | <0.01 |
| RARα-LIG | 0.67 ± 0.04 | 0.22 ± 0.01 | 2.37 ± 0.04 | 0.05 | <0.01 | 0.01 |
| RARγ-LIG | 0.81 ± 0.04 | 0.22 ± 0.01 | 2.64 ± 0.01 | 0.20 | <0.01 | <0.01 |
| RXR-LIG | 0.64 ± 0.04 | 0.51 ± 0.04 | 1.04 ± 0.04 | 0.05 | 0.03 | 0.14 |
Expression after 24 h in cultured 3T3-L1 adipocytes with ATRA (1 μM), an RARα-specific agonist BMS753/RARα-LIG (1 μM), an RARγ-specific agonist BMS189961/RARγ-LIG (1 μM), and an RXR ligand RXR-LIG/LG268 (1 μM) calculated with CTRL treatments set as 1. Significance and sem are based on n = 6 parallel treatments. Significant values vs. CTRL are in italics. LIG, ligand. REX, relative expression.