Figure 2.

miR-184 inhibits angiogenesis in the Matrigel plug assay in vivo. Effects of miR-184 on angiogenesis were assessed by immunofluorescence staining of Matrigel plugs using a CD31 antibody. A, B) Representative images of CD31 staining in Matrigel plugs containing CM from HLEKs expressing negative control miR (control) or miR-184 (A) and conditioned medium from HCEKs after treatment with irrelevant antagomir (ir-antago, control) or antago-184 (B). Bar graphs show quantification analysis of CD31-positive cells in sections, and the data are expressed as percentage values under input control (d 5), which was assigned a value of 100. *P < 0.05; **P < 0.01.