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. 2016 Oct 7;31(1):256–265. doi: 10.1096/fj.201600746R

Figure 3.

Figure 3.

miR-184 targets FOG2, PDGF-β, and PPAP2B. A) Screening the interactions of FOG2, PDGF-β, and PPAP2B using the psiCHECK-2 vector harboring 3′UTR of each gene. Constructs were cotransfected with either miR-control or miR-184 into HLEKs. Twenty-four hours after transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System. N, precursor miRNA control; 184, precursor miR-184. **P < 0.01. B) Immunoblotting of FOG2, PDGF-β, and PPAP2B in HLEKs after transfection of precursor miRNA negative control (N) or precursor miR-184 (184). C–G) Tissue immunofluorescence staining with FOG2 (C), PDGF-β (D), and PPAP2B (E) antibodies in the frozen section of human limbal and corneal epithelium. No specific staining was observed in the limbus and cornea stained with isotype control antibodies, rabbit monoclonal antibody IgG (F), and rabbit polyclonal antibody IgG (G).