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. 2016 Oct 17;31(1):412–420. doi: 10.1096/fj.201600954R

Figure 3.

Figure 3.

Runx2 mediated goblet cell differentiation by regulating SPDEF expression. A, B) Knockdown of Runx2 inhibited IL-3-induced SPDEF and mucin 5AC expression. Serum-starved NHBECs were transduced with Ad-GFP or Ad-shRunx2, followed by treatment with vehicle or IL-13 (50 ng/ml) for 2 d. Western blot analysis was performed to examine expression of proteins as indicated. B) Quantification of SPDEF expression shown by normalizing to α-tubulin level. *P < 0.01 compared to Ad-GFP group without IL-13; #P < 0.01 compared to Ad-GFP group with IL-13 (n = 3). C–E) Runx2 promoted SPDEF protein expression in dose-dependent manner. NHBECs were transduced with Ad-GFP (7 × 109 PFU/ml, 2 µl/ml) or Ad-Runx2 (3 × 1010 PFU/ml: 0.5 µl/ml (+), 1 µl/ml (++), 2 µl/ml (+++)) for 3 d. Western blot analyses (C) were performed to examine Runx2 and SPDEF expression. D, E) Quantification of Runx2 (D) and SPDEF (E) protein expression shown in C by normalizing to α-tubulin level. *P < 0.01 compared to Ad-GFP group for corresponding proteins (n = 3).