Skip to main content
. 2016 Oct 19;31(1):346–355. doi: 10.1096/fj.201600560R

Figure 5.

Figure 5.

Effects of PKD1 and IFT88 siRNA knockdown on hASC primary cilia. A, B) Gene expression of cilia-associated structural proteins, PKD1 (A) and IFT88 (B). C) Percentage of hASCs observed with cilia structure. D–F) Representative fluorescence images of hASC primary cilia after negative control siRNA (D), PKD1 siRNA (E), and IFT siRNA knockdown (F). White arrows indicate the immunostained primary cilia observed under fluorescent microscope. RNA extraction and immunostaining were performed after 72 h culture in ODM after siRNA knockdown. mRNA expressions were normalized to negative control siRNA transfected samples. Error bars = sem, Student’s t test compared siRNA knockdown samples with negative control siRNA-treated samples. *P < 0.05.