Figure 6.

Enhancement of OCT3/4 Binding by PRDM14 during Reversion from Primed to Naive Pluripotency

(A) Comparison of OCT3/4 binding peaks between ESCs and EpiLCs (d2), EpiLCs (d2) and EpiLCs + PRDM14, and EpiLCs + PRDM14 and ESCs. Numbers in gray rectangles indicate peaks common to both cell types in each pair. Numbers in blue, red, and green rectangles indicate peaks specific to ESCs, EpiLCs (d2), and EpiLCs + PRDM14, respectively.

(B) Genes downregulated by PRDM14 during the transition from EpiLCs to ESCLCs (red circles). Genes associated with PRDM14 and OCT3/4 binding that were enhanced during the transition of EpiLCs to ESCs are indicated by navy circles; genes upregulated by PRDM14 in EpiLCs are shown by yellow circles.

(C) GO annotation of genes upregulated by PRDM14 in which OCT3/4 binding increased with PRDM14 binding (from EpiLCs to EpiLCs + PRDM14).

(D) ChIP-seq tracks of OCT3/4 and PRDM14 in ESCs, EpiLCs (d2), and EpiLCs + PRDM14. Intensity values of microarray data are shown on the right side. Red bar indicates the position of the primers for ChIP-qPCR and GlucMS-qPCR.

(E) ChIP-qPCR analysis of OCT3/4 and PRDM14 expression in ESCs, EpiLCs (d2), and EpiLCs + PRDM14. Fold enrichment indicates values of each IgG relative to input values using the same amount of DNA as template. Error bars indicate ±SD of a technical duplicate.

(F) GlucMS-qPCR analysis of the percentage of 5mC and 5hmC marks at pluripotency genes. Error bars indicate ±SD of a technical duplicate.