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Isothermal PCR reaction with SYBR green.
From left to right, reactions with input of 100ng, 10ng, 1ng, and 100pg K562 RNA demonstrate grossly visible change in color indicating positive fusion signals after one hour at 63°c. The mid right two reactions contained 10pg and 1pg of K562 RNA did not show an obvious grossly visible change in color. The two far right reactions, have no K562 RNA and show no color change.
