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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Nucl Med Biol. 2016 Sep 3;44:4–30. doi: 10.1016/j.nucmedbio.2016.08.015

Fig. 9.

Fig. 9

Panel A: Saturation binding of [125I]-E-IA-BF-PE-PIPZE (0.016 – 1.6 nM) to mouse brain membranes (0.15 mg protein / mL) at 37 °C with a 180 min incubation and haloperidol (1.0 μM) to define non-specific binding. Panel B: Traditional Rosenthal plot for visualization of the relationship between bound radioligand, and the ratio of bound radioligand to free radioligand. Data are from a representative experiment performed in duplicate, and replicated four times to give Kd 0.24 ± 0.01 nM and Bmax 472 ± 13 fmol / mg protein by non-linear regression.