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Diazo compounds for covalent modification of proteins. (A) Putative mechanism for the esterification of carboxylic acids with a diazo compound,134 and its application to the bioreversible labeling of a protein.128,129 Diazo compound I is optimized for protein esterification.129 (B) Putative mechanism of a diazo carbenoid insertion reaction, and its application to the site-specific modification of a proximal amino-acid residue.161
