Figure 3. Hindpaw threshold sensitivity throughout the 18-day timecourse remains stable.

(A, B) Compared to hindpaw threshold responses relative to BL levels in sham-treated rats, CCI leads to robust bilateral and persistent allodynia that is unaltered by wheel running activity occurring during the inactive portion of the diurnal cycle. No hindpaw threshold differences between CCI – locked wheel and CCI – unlocked wheel throughout the timecourse were observed. Tukey’s test for post-hoc contrasts between each group at each time were performed, and Šidák’ post-hoc comparisons were performed between each CCI group at each time. ** P < 0.001. (C, D) This separate group of rats replicated our prior results of bilateral hindpaw sensitivity following CCI compared to sham controls, which verifies (1) stable ongoing allodynia was present in rats that were also examined for wheel running activity during the active phase of the diurnal cycle, and (2) this paradigm of acute exposure to active-cycle voluntary wheel running activity does not alter the pattern of ongoing allodynia. Šidák’ post-hoc comparisons were performed between CCI and sham groups at each time. # P < 0.01.