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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Photochem Photobiol. 2016 Oct 17;92(6):842–853. doi: 10.1111/php.12636

Figure 1.

Figure 1

Localization of PpIX in ALA-treated U87 cells. Cells grown to ~40 % confluence on cover slips were dark-incubated with 1 mM ALA in serum-free medium for 30 min. After a switch to ALA-free medium, the cells were treated with 1 μM Rhodamine-123 (Rh123) for 20 min, washed and immediately examined by confocal fluorescence microscopy. Excitation and emission wavelengths for PpIX were 488 nm and 620–650 nm, respectively; those for Rh123 were 505 nm and 530–540 nm, respectively. Scale bar: 25 μm.