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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Photochem Photobiol. 2016 Oct 17;92(6):842–853. doi: 10.1111/php.12636

Figure 3.

Figure 3

Apoptotic photokilling of U87 cells and effects of NOS inhibitors. U87 cells at 40–45% confluence were treated with 1 mM ALA without or with a NOS inhibitor (25 μM 1400W or 1 mM L-NAME), and then irradiated (1 J/cm2). After 4 h of follow-up dark incubation, the cells were treated with Annexin V-FITC and Propidium Iodide (PI) according to supplier recommendations. Camptothecin (CPT, 25 μM) served as a maximum apoptosis standard. Extent of staining was quantified using a plate reater with excitation/emission wavelengths of 485/535 nm for Annexin V-FITC and 560/595 nm for PI. Less than 5% PI staining was detected for any of the treatment conditions.