Figure 1. Long non-coding RNA Morrbid is a critical regulator of eosinophils, neutrophils, and Ly6Chi monocytes.
(a) Human neutrophil and mouse granulocyte normalized RNA-seq and ChIP-seq tracks at the Morrbid locus.
(b) Single molecule Morrbid RNA FISH.
(c) qPCR expression of mouse (n=3; representative of 3 independent experiments) and
(d) human Morrbid in indicated cell-types and tissues (n=7).
(e) WT and Morrbid-deficient flow-cytometry plots and absolute counts (n=3-5; representative of 7 independent experiments).
(f-g) L. monocytogenes infection of WT and Morrbid-deficient mice. (f) Survival and weight loss (n=9, representative of 3 independent experiments). (g) CFUs/g from indicated organs (n=5; representative of 3 independent experiments).
Error bars show s.e.m. *p < 0.05, **p < 0.01, and ***p < 0.001 (two-sided t-test e,g, (right) f; Mantel-Cox test (left) f).