Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Inorg Biochem. 2016 Oct 17;166:26–33. doi: 10.1016/j.jinorgbio.2016.10.007

Figure 2.

Figure 2

Steady-state kinetics of FIH variants with CTAD as the varied substrate. WT (■) D201G (●) Inset: D201E (▼) D201A (◆). All assays contained saturating concentrations of FeSO4 (25 μM), αKG (100 μM), and ascorbate (2 mM), with an ambient O2 concentration (217 μM) and were performed in 50 mM HEPES containing 100 mM NaCl, pH 7.00, 37°C.