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Autohydroxylation following exposure to air of FIH variants (100 μM) mixed anaerobically with FeSO4 (100 μM) and αKG (500 μM) in 50 mM HEPES pH 7.00 containing 100 mM NaCl. (A) Timecourse for WT-FIH and the D201E variant; inset, D201E spectra. (B) Timecourse for the D201G variant; inset, D201G spectra.
