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O2 Activation Kinetic parameters for FIH and Asp201 variantsa.
| kcatapp (min−1) | kcat/KM(O2)app (μM−1 min−1) | KM(O2)app (μM) | |
|---|---|---|---|
| WT FIHb | 33 ± 3 | 0.17 ± 0.03 | 200 ± 40 |
| D201E | 0.81 ± 0.02 | 0.014 ± 0.002 | 70 ± 15 |
| D201G | 20 ± 1.0 | 0.18 ± 0.02 | 115 ± 20 |
Reactions contained ascorbate (2 mM), αKG (100 μM), FeSO4 (25 μM), CTAD (80 μM) and O2 (0–950 μM) in 50 mM HEPES containing 100 mM NaCl pH 7.00, 37.0 °C.
From Ref. ([25])
