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Coupling ratios for facial triad variants of FIH.
| Variant | C x kcat/KM(O2)app (μM−1 min−1) | kcat/KM(O2)app (μM−1 min−1) | C |
|---|---|---|---|
| WT (Asp201) | 0.18 ± 0.03 | 0.17 ± 0.03 | 1.05 ± 0.05 |
| D201G | 0.36 ± 0.05 | 0.18 ± 0.02 | 2.0 ± 0.20 |
| D201A | ---- | ---- | ≫ 80a |
| D201E | 0.28 ± 0.06 | 0.014 ± 0.002 | 20 ± 3 |
C = (moles succinate)/(moles CTAD-OH)
Estimated based on moles of succinate produced and the limit of detection for hydroxylation.
