Skip to main content
. Author manuscript; available in PMC: 2017 Dec 16.
Published in final edited form as: ACS Synth Biol. 2016 Jul 22;5(12):1475–1484. doi: 10.1021/acssynbio.6b00097

Figure 5. Full and partial agonism of β-gly W33G.

Figure 5

Enzyme assays carried out at high substrate concentration (750 μM FDGal) demonstrate the dependence of the initial velocity (and thus Vmax) on effector concentration. Both panels show the same data, expanded in the right panel to better show the agreement between the data and the model that was fit to it. All data were collected in triplicate, with all replicates shown explicitly on the plots. Experimental uncertainly of the fit values for KD and W are reported as a range corresponding to one standard error in either direction.