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. Author manuscript; available in PMC: 2017 Dec 16.
Published in final edited form as: ACS Synth Biol. 2016 Jul 22;5(12):1475–1484. doi: 10.1021/acssynbio.6b00097

Figure 7. N-methylaniline is the optimal rescuing ligand for β-gly W33A.

Figure 7

The relatively modest rescue by N-methylaniline, as well as the fact that 3-methylindole rescued better than indole, can be explained by separately examining the binding affinity (KD) and contribution to the active site geometry (W) for each compound. In all cases 750 μM FDGal was used as substrate. All data were collected in triplicate, with all replicates shown explicitly on the plots. Experimental uncertainly of the fit values for KD and W are reported as a range corresponding to one standard error in either direction.