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. Author manuscript; available in PMC: 2017 Dec 8.
Published in final edited form as: Photochem Photobiol. 2016 Dec 8;92(6):816–825. doi: 10.1111/php.12659

Figure 4. Resatorvid inhibits UV-induced acute inflammatory signaling and potentiates cell death in mouse epidermis.

Figure 4

SKH-1 mice were treated with an acute dose of SSL (105 kJ/m2 UVA/6.4 kJ/m2 UVB) and post-treated topically with 0.5% resatorvid or acetone (vehicle). 24 hr later, mice were sacrificed and back skins were harvested. (A) Epidermal protein lysates were analyzed by immunoblot analysis for UV-induced phosphorylation of p38 and p65/NF-κB. (B) qRT-PCR of epidermal RNA shows induction of IL-10 mRNA which is significantly inhibited by resatorvid. (C) Skins were sectioned for IHC and stained for cleaved caspase 3, 600× magnification is shown. (D) Quantification of stained cells per field in 400× images from skins in C (p < 0.05). (E) HaCaT keratinocytes were pretreated with 10 µM resatorvid for 1 hr prior to SSL treatment (40 kJ/m2 UVA/ 2.7kJ/m2 UVB) and post-treated with the same dose of the compound. Cells were harvested 18 hr later for analysis of cell death using annexin V/PI staining via flow cytometry.