Extended Data Figure 1. Pharmacokinetic profile of GNF6702 in mouse.

a, Time profiles of mean free plasma concentration of GNF6702 in mouse model of visceral leishmaniasis; free GNF6702 concentration values were predicted from measured total plasma concentration values collected on day 1 and day 8 of treatment. Dashed blue lines correspond to intra-macrophage L. donovani EC₅₀ of 18 ± 1.8 nM and EC₉₉ of 42 ± 5.6 nM. Circles: means ± s.d.; n=3 mice for treatment day 1; n=5 mice for treatment day 8; fraction unbound in mouse plasma=0.063. For data points lacking error bars, standard deviations are smaller than circles representing means. b, Time course of total GNF6702 concentration in mouse plasma and brain after single oral dose (20 mg/kg); n=2 mice per time point; circles: measured values; rectangles: means.