Extended Data Figure 2. GNF6702 clears parasites from mice infected with T. brucei.

a, In vivo quantification of bioluminescent T. brucei in infected mice before and after treatment. ip: intraperitoneal; day 21: start of treatment; day 28: 24 hours after last GNF6702 dose; day 42: evaluation of early parasite recrudescence in mice treated with diminazene aceturate (n=3); day 42 and 92: absence of parasite recrudescence in mice treated with GNF6702 (n=6). Images from uninfected mice (3 mice of 4 are shown) aged-matched for day 0 were collected independently using the same acquisition settings. Parasitemia (blue font) and whole mouse total flux (black font) values of each animal are shown above the image; N.D.: not detectable. Within each group the mouse numbers in yellow (top left in each image) refer to the same mouse imaged throughout. Complete sets of parasitemia and whole mouse total flux values collected on individual mice throughout the experiment are listed in Supplementary Tables 4 and 5. b, Brains from mice shown in panel a were soaked in luciferin and imaged for presence of bioluminescent T. brucei at the indicated time points. For three diminazene-treated mice, two images of each brain are shown, one at a lower sensitivity (left) and the other at a high signal intensity scale.