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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Nature. 2016 Aug 8;537(7619):229–233. doi: 10.1038/nature19339

Extended Data Figure 4. Mutations in proteasome beta 4 subunit confer resistance to GNF6702 in T. cruzi and T. brucei.

Extended Data Figure 4

a, growth curves of wild type, GNF3943-resistant and GNF8000-resistant T. cruzi epimastigote strains in the presence of increasing concentrations of GNF6702, nifurtimox, bortezomib and MG132; RU (relative units) corresponds to parasite growth relative to the DMSO control (%); for data points lacking error bars, standard errors are smaller than circles representing means; due to limited aqueous solubility, the highest tested GNF6702 concentration was 10 μM. b, growth inhibition EC50 values of GNF6702, bortezomib, MG132 and nifurtimox on indicated T. cruzi strains. c, growth inhibition EC50 values of GNF6702 and bortezomib on T. cruzi epimastigotes and T. brucei bloodstream form trypomastigotes overexpressing PSMB4WT or PSMB4F24L. Data shown in panels a, b and c correspond to means ± s.e.m. (n=3 technical replicates).