Skip to main content
. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Nature. 2016 Sep 21;538(7623):60–65. doi: 10.1038/nature19757

Extended Data Figure 1. Structure determination for the Ton subcomplex (ExbB/ExbDΔperi) using Se-SAD at 5.2 Å resolution.

Extended Data Figure 1

A. The initial structure of the Ton subcomplex was solved by Se-SAD using anisotropic data extending to 5.2 Å resolution. The data from two crystals was processed with Xia2 and the initial sites found using HKL2MAP v0.3, which found a single solution every ~10,000 tries; resolution limits were also important for finding a solution. B. The sites were then input into AUTOSOL/PHENIX for site refinement and density modification, producing density maps (blue mesh) which clearly showed 5-fold symmetry and allowed an initial model of a monomer to be built, consisting almost entirely of α-helices. This model was then used as a search model for molecular replacement to solve the 2.6 Å native structure (data obtained from a single crystal). C. Anomalous different map (orange mesh) showing density for the selenium sites in the 5.2 Å Se-incorporated structure.