Figure 3.

Assignment of the D₃ state of TF as the 70S-ribosome-bound state. (A) Comparison of fractional populations of the three diffusion states of TF^mE in the chromosomally-tagged strain (i.e., TF^mE), further overexpressed from a pBAD24 plasmid (i.e., TF^mE(p)), with the FRK/AAA mutations expressed from a pBAD24 plasmid in the $\mathrm{\Delta }$tig knockout strain (i.e., ${\text{TF}}_{\text{FRK}/\text{AAA}}^{\text{mE}}$(p)), or in the strain treated with 200 μM rifampicin (i.e., TF^mE + Rif). (B) Probability distribution difference of pairwise distances of initial TF^mE tracking positions relative to the simulated uniform position distributions in the cell. Other positions: the positions excluding those of the thresholded D₃ state positions. (C) Probability distribution of the initial TF^mE tracking positions along the cell short axis.