Figure 4.

Assignment of the D₂ state of TF. (A) Comparison of the value of diffusion constant D₂ of TF^mE in the chromosomally-tagged strain (TF^mE), further overexpressed from a pBAD24 plasmid (i.e., TF^mE(p)), containing the FRK/AAA mutations expressed from a pBAD24 plasmid in the $\mathrm{\Delta }$tig strain ( ${\text{TF}}_{\text{FRK}/\text{AAA}}^{\text{mE}}$(p)), chromosomally-tagged in $\mathrm{\Delta }$dnaK strain ( ${\text{TF}}_{\mathrm{\Delta }\text{dnaK}}^{\text{mE}}$), and chromosomally-tagged in $\mathrm{\Delta }$dnaJ strain ( ${\text{TF}}_{\mathrm{\Delta }\text{dnaJ}}^{\text{mE}}$). (B) Comparison of fractional populations of the three diffusion states of TF^mE, ${\text{TF}}_{\mathrm{\Delta }\text{dnaK}}^{\text{mE}}$, and ${\text{TF}}_{\mathrm{\Delta }\text{dnaJ}}^{\text{mE}}$.