Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Microbiol. 2016 Oct 17;102(6):1075–1085. doi: 10.1111/mmi.13537

FIGURE 3. ClpAP degradation of DnaA is crucial when Lon is compromised.

FIGURE 3

A. Morphology and doubling time of cells lacking ClpS, ClpA and Lon (ΔlonΔclpS-clpA), either without (−van) or with (+van) vanillate induced expression of ClpA from a low copy plasmid (pRVMCS-2 Pvan-clpA). B. Steady state levels of ClpA, DnaA and ClpP in these strains. *: cross-reacting band. **: ClpA (note leaky expression in absence of inducer) (n = 2; error bars represent SD). C. Flow cytometry profiles showing chromosome content of ΔlonΔclpS-clpA, with or without vanillate induced expression of ClpA. D. DnaA degradation upon translation shutoff in ΔlonΔclpS-clpA cells either with or without vanillate induced expression of ClpA.