FIGURE 5. Lon degrades DnaAR357A poorly.
A. Comparison of DnaA and DnaAR357A degradation by Lon and ClpAP (n= 3; error bars represent SD). B. Degradation of denatured DnaA and DnaAR357A by Lon and ClpAP (n= 3; error bars represent SD). C. In vivo degradation of DnaA or DnaAR357A following translation shutoff by chloramphenicol. DnaAR357A was expressed in a strain harboring a functional DnaA-YFP fusion to allow for resolution of wild type and mutant variants.