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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Microbiol. 2016 Oct 17;102(6):1075–1085. doi: 10.1111/mmi.13537

FIGURE 6. ClpA protects cells from over-activation of DnaA.

FIGURE 6

A. Wildtype or mutant strains carrying inducible dnaA on a medium copy plasmid were induced for 6 hours (0.2% xylose). Cells were serially diluted, then plated on PYE agar without inducer. B. Protein levels in wildtype, ΔclpS and ΔclpA strains after 6 hours of DnaA induction and after an additional 6 hours following removal of the inducer. C. Wildtype or mutant strains carrying dnaA or dnaAR357A on a low copy plasmid were serially diluted and plated on PYE agar with 0.2% xylose. D. Levels of DnaA in wildtype, ΔclpS and ΔclpA strains during growth where DnaAR357A is continuously induced from a low copy plasmid. Inset compares DnaA levels directly across all three strains and shows ClpP as a control.