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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Exp Eye Res. 2016 Jun 16;158:43–50. doi: 10.1016/j.exer.2016.06.006

Fig. 1.

Fig. 1

Differential dendritic shrinkage and loss of synapses between ON and OFF αRGCs after intraocular pressure elevation. Biolistically labeled alpha RGCs (αRGCs) in control retinas (first column) and 3 or 30 days after laser-induced ocular hypertension in mice (second and third column, respectively). Cell morphology was visualized by cytosolic transfection of tdTomato (red), while excitatory post-synaptic sites on dendrites were labeled by co-transfection of the cell with PSD95-YFP (green). Dendritic stratification was visualized in the orthogonal views by immunolabeling against Synaptotagmin 2 (Syt2), a marker of Type 2 (T2) OFF and Type 6 (T6) ON bipolar cells. Despite similar dendritic arbor size in control retinas, OFF αRGCs undergo greater dendritic and synaptic degeneration compared to ON αRGCs at both 3 and 30 days after intraocular pressure elevation. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)