Extended Data Figure 8. Projection mapping and retrograde tracing from SFO neurons.

a, Schematic of viral strategy for identifying projections from SFO^Nos1 neurons using a fluorescent synaptophysin fusion protein. b, Representative images showing SFO^Nos1 neuron somas in the SFO and axon terminals in the organum vasculosum of the lamina terminalis (OVLT), median preopotic nucleus (MnPO), paraventricular hypothalamus (PVH), and supraoptic nucleus (SON) (1 of 2 mice; green, GFP; blue, DAPI; scale bars, 100 µm). c, Schematic of strategy for retrograde tracing from SFO neurons using retrobeads. d, Representative images showing retrobeads injection site in the SFO and retrograde-labeled neurons in the medial septum (MS), OVLT, MnPO, arcuate nucleus (ARC), median raphe (MnR), dorsal raphe (DR), and locus coeruleus (LC) (1 of 2 mice; red, rhodamine; blue, DAPI; scale bars, 100 µm).